Cyclometalated Au^III Complexes for Cysteine Arylation in Zinc Finger Protein Domains: towards Controlled Reductive Elimination.

ABSTRACT: With the aim of exploiting the use of organometallic species for the efficient modification of proteins through C-atom transfer, the gold-mediated cysteine arylation through a reductive elimination process occurring from the reaction of cyclometalated Au^III C^N complexes with a zinc finger peptide (Cys₂ His₂ type) is here reported. Among the four selected Au^III cyclometalated compounds, the [Au(C^CO N)Cl₂ ] complex featuring the 2-benzoylpyridine (C^CO N) scaffold was identified as the most prone to reductive elimination and Cys arylation in buffered aqueous solution (pH 7.4) at 37 °C by high-resolution LC electrospray ionization mass spectrometry. DFT and quantum mechanics/molecular mechanics (QM/MM) studies permit

SUBMITTER: Wenzel MN 

PROVIDER: S-EPMC6594228 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature