A High-Throughput Fluorescence-Based Assay for Rapid Identification of Petroleum-Degrading Bacteria.

ABSTRACT: Over the past 100 years, oil spills and long-term waste deposition from oil refineries have significantly polluted the environment. These contaminants have widespread negative effects on human health and ecosystem functioning. Natural attenuation of long chain and polyaromatic hydrocarbons is slow and often incomplete. Bioaugmentation of polluted soils with indigenous bacteria that naturally consume petroleum hydrocarbons could speed up this process. However, the characterization of bacterial crude oil degradation efficiency - which often relies upon expensive, highly specialized gas-chromatography mass spectrometry analyses - can present a substantial bottleneck in developing and implementing these bioremediation strategies. Here, we develop a low-cost, rapid, high-throughput fluorescence

SUBMITTER: French KE 

PROVIDER: S-EPMC6594354 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature