Integrating 3D Cell Culture of PC12 Cells with Microchip-Based Electrochemical Detection.

ABSTRACT: Developing in vitro cell culture models that accurately mimic in vivo processes in a manner that also enables near real-time analysis of neurotransmitters is an important research area. New technologies being developed such as 3D scaffolds for cell culture and 3D printed microfluidics provide an opportunity for such advancements. In this work, PC12 cells were used as a model system and they were immobilized onto a 3D scaffold of polystyrene (PS) fibers. These fibers were created by electrospinning onto PS sheets, which were laser cut and, after cell seeding, inserted into a 3D printed microfluidic device. The 3D printed device was designed with threads for connecting commercial fittings (to integrate automated pumps and a 4-port injection system) and a steel pin for simple co

SUBMITTER: Mehl BT 

PROVIDER: S-EPMC6594695 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature