APRIL/BLyS Blockade Reduces Donor-specific Antibodies in Allosensitized Mice.

ABSTRACT:

Background

Highly sensitized candidates on the transplant waitlist remain a significant challenge, as current desensitization protocols have variable success rates of donor-specific antibody (DSA) reduction. Therefore, improved therapies are needed. A proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS) are critical survival factors for B-lymphocytes and plasma cells, which are the primary sources of alloantibody production. We examined the effect of APRIL/BLyS blockade on DSA in a murine kidney transplant model as a possible novel desensitization strategy.

Methods

C57BL/6 mice were sensitized with intraperitoneal (IP) injections of 2 × 10 BALB/c splenocytes. Twenty-one days following sensitization, animals were treated with 100 μg of BLyS blockade (B-cel

SUBMITTER: Wilson NA 

PROVIDER: S-EPMC6594891 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature