Identification and in silico characterization of cis-acting elements of genes involved in carotenoid biosynthesis in tomato.

ABSTRACT: Carotenoids, the widespread and structurally diverse class of pigments, accumulate in the fruits of tomato plants in a tissue specific manner. The carotenoid biosynthetic pathway genes have been cloned and characterized in tomato and other plants, however, its regulation is still obscure. We collected and analyzed forty different accessions of tomato for the present study. HPLC analysis revealed differential accumulation of major carotenoids (lycopene and ß-carotene) in the ripe fruit tissue. In order to understand the underlying regulatory mechanisms in carotenoid biosynthesis and accumulation, we sequenced the cis-acting elements i.e. promoter, 5' and 3' untranslated regions of the carotenoid pathway genes, in all accessions, followed by their in silico validation. Major differenc

SUBMITTER: Koul A 

PROVIDER: S-EPMC6595038 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature