MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation.

ABSTRACT:

Purpose

To elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function.

Materials and methods

Exosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering). MicroRNA (miRNA) microarray was performed to detect the expression of miRNAs in 3 exosome samples. Results revealed miRNAs that were simultaneously expressed in the 3 exosome samples and were previously reported to exist in mature RBCs. Functions and potential pathways of some detected miRNAs were illustrated by bioinformatic analysis. Validation of the top 3 abundant miRNAs was carried out

SUBMITTER: Huang H 

PROVIDER: S-EPMC6595350 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature