A Microsatellite Multiplex Assay for Profiling Pig DNA in Mosquito Bloodmeals.

ABSTRACT: Genetic profiling has been used to link mosquito bloodmeals to the individual humans, but this analysis has not been done for other mammalian bloodmeals. In this study, we describe a microsatellite-based method for identifying individual pigs in mosquito bloodmeals based on their unique multilocus genotypes. Eleven tetranucleotide microsatellites and a sex-specific marker were selected based on Smith-Waterman DNA sequence alignment scores from the reference genome and primers were designed with features that reduce primer dimers, promote complete adenylation, and enable fluorescent labeling of amplicons. A multiplex polymerase chain reaction (PCR) assay was optimized and validated by analyzing DNA of individual pigs from several nuclear families and breeds before it was used to analyze gen

SUBMITTER: Keven JB 

PROVIDER: S-EPMC6595529 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature