The effect of AP-2δ on transcription of the Prestin gene in HEI-OC1 cells upon oxidative stress.

ABSTRACT:

Background

The study aimed to investigate the effect of oxidative stress on Prestin expression, and explore the transcription factors (TFs) that are involved in regulating the expression of Prestin in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells upon oxidative stress.

Methods

Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression level of Prestin. Reverse chromatin immunoprecipitation (reverse ChIP) assay was performed to identify proteins that could bind to the Prestin gene. Small interfering RNA (siRNA) and chromatin immunoprecipitation (ChIP) experiments were used to further verify the results. HEI-OC1 cells were incubated with four different concentrations of tert-butyl hydroperoxide (t-BHP) for 24 h

SUBMITTER: Luo X 

PROVIDER: S-EPMC6595603 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature