Proteomic changes in the xylem sap of Brassica napus under cadmium stress and functional validation.

ABSTRACT:

Background

The xylem sap of vascular plants primarily transports water and mineral nutrients from the roots to the shoots and also transports heavy metals such as cadmium (Cd). Proteomic changes in xylem sap is an important mechanism for detoxifying Cd by plants. However, it is unclear how proteins in xylem sap respond to Cd. Here, we investigated the effects of Cd stress on the xylem sap proteome of Brassica napus using a label-free shotgun proteomic approach to elucidate plant response mechanisms to Cd toxicity.

Results

We identified and quantified 672 proteins; 67% were predicted to be secretory, and 11% (73 proteins) were unique to Cd-treated samples. Cd stress caused statistically significant and biologically relevant abundance changes in 28 xylem sap proteins. Among th

SUBMITTER: Luo JS 

PROVIDER: S-EPMC6595625 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature