Steady-State Free Ca²⁺ in Astrocytes Is Decreased by Experience and Impacts Arteriole Tone.

ABSTRACT: Astrocytes can control basal synaptic strength and arteriole tone via their resting Ca²⁺ activity. However, whether resting astrocyte Ca²⁺ can adjust to a new steady-state level, with an impact on surrounding brain cells, remains unknown. Using two-photon Ca²⁺ imaging in male rat acute brain slices of the somatosensory neocortex, we found that theta burst neural activity produced an unexpected long-lasting reduction in astrocyte free Ca²⁺ in the soma and endfeet. The drop in intracellular Ca²⁺ was attenuated by antagonists targeting multiple ionotropic and metabotropic glutamate receptors, and intracellular cascades involved Ca²⁺ stores and nitric oxide. The reduction in astrocyte endfoot Ca²⁺ was coincident with

SUBMITTER: Mehina EMF 

PROVIDER: S-EPMC6596783 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature