A collection of yeast cellular electron cryotomography data.

ABSTRACT:

Background

Cells are powered by a large set of macromolecular complexes, which work together in a crowded environment. The in situ mechanisms of these complexes are unclear because their 3D distribution, organization, and interactions are largely unknown. Electron cryotomography (cryo-ET) can address these knowledge gaps because it produces cryotomograms-3D images that reveal biological structure at ∼4-nm resolution. Cryo-ET uses no fixation, dehydration, staining, or plastic embedment, so cellular features are visualized in a life-like, frozen-hydrated state. To study chromatin and mitotic machinery in situ, we subjected yeast cells to genetic and chemical perturbations, cryosectioned them, and then imaged the cells by cryo-ET.

Findings

Here we share >1,000 cryo-ET raw data

SUBMITTER: Gan L 

PROVIDER: S-EPMC6596884 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature