Development and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections.

ABSTRACT: Soil-transmitted helminths (STH) are a major cause of morbidity in tropical developing countries with a global infection prevalence of more than one billion people and disease burden of around 3.4 million disability adjusted life years. Infection prevalence directly correlates to inadequate sanitation, impoverished conditions and limited access to public health systems. Underestimation of infection prevalence using traditional microscopy-based diagnostic techniques is common, specifically in populations with access to benzimidazole mass treatment programs and a predominance of low intensity infections. In this study, we developed a multiplexed-tandem qPCR (MT-PCR) tool to identify and quantify STH eggs in stool samples. We have assessed this assay by measuring infection prevalence and inte

SUBMITTER: Stracke K 

PROVIDER: S-EPMC6597125 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature