Utility of a ready-to-use PCR system for neuroendocrine tumor diagnosis.

ABSTRACT:

Background

Multigene-based PCR tests are time-consuming and limiting aspects of the protocol include increased risk of operator-based variation. In addition, such protocols are complex to transfer and reproduce between laboratories.

Aims

Evaluate the clinical utility of a pre-spotted PCR plate (PSP) for a novel multigene (n = 51) blood-based gene expression diagnostic assay for neuroendocrine tumors (NETs).

Methods

A pilot study (n = 44; 8 controls and 36 NETs) was undertaken to compare CQ, normalized gene expression and algorithm-based output (NETest score). Gene expression was then evaluated between matched blood:tumor tissue samples (n = 7). Thereafter, two prospective sets (diagnostic: n = 167; clinical validation: n = 48, respectively) were evaluated for diagnost

SUBMITTER: Kidd M 

PROVIDER: S-EPMC6597157 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature