A distal super enhancer mediates estrogen-dependent mouse uterine-specific gene transcription of <i>Igf1</i> (<i>insulin-like growth factor 1</i>).

ABSTRACT: Insulin-like growth factor 1 (IGF1) is primarily synthesized in and secreted from the liver; however, estrogen (E2), through E2 receptor α (ERα), increases uterine Igf1 mRNA levels. Previous ChIP-seq analyses of the murine uterus have revealed a potential enhancer region distal from the Igf1 transcription start site (TSS) with multiple E2-dependent ERα-binding regions. Here, we show E2-dependent super enhancer-associated characteristics and suggest contact between the distal enhancer and the Igf1 TSS. We hypothesized that this distal super-enhancer region controls E2-responsive induction of uterine Igf1 transcripts. We deleted 430 bp, encompassing one of the ERα-binding sites, thereby disrupting interactions of the enhancer with gene-regulatory factors. As a res

SUBMITTER: Hewitt SC 

PROVIDER: S-EPMC6597841 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature