The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies.

ABSTRACT: Pinaceae are the predominant photosynthetic species in boreal forests, but so far no detailed description of the protein components of the photosynthetic apparatus of these gymnosperms has been available. In this study we report a detailed characterization of the thylakoid photosynthetic machinery of Norway spruce (Picea abies (L.) Karst). We first customized a spruce thylakoid protein database from translated transcript sequences combined with existing protein sequences derived from gene models, which enabled reliable tandem mass spectrometry identification of P. abies thylakoid proteins from two-dimensional large pore blue-native/SDS-PAGE. This allowed a direct comparison of the two-dimensional protein map of thylakoid protein complexes from P. abies with the model angiosperm Arabidopsis

SUBMITTER: Grebe S 

PROVIDER: S-EPMC6598058 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature