Composition of the Biofilm Matrix of Cutibacterium acnes Acneic Strain RT5.

ABSTRACT: In skin, Cutibacterium acnes (former Propionibacterium acnes) can behave as an opportunistic pathogen, depending on the strain and environmental conditions. Acneic strains of C. acnes form biofilms inside skin-gland hollows, inducing inflammation and skin disorders. The essential exogenous products of C. acnes accumulate in the extracellular matrix of the biofilm, conferring essential bacterial functions to this structure. However, little is known about the actual composition of the biofilm matrix of C. acnes. Here, we developed a new technique for the extraction of the biofilm matrix of Gram-positive bacteria without the use of chemical or enzymatic digestion, known to be a source of artifacts. Our method is based on the physical separation of the cells

SUBMITTER: Gannesen AV 

PROVIDER: S-EPMC6598116 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature