Bacterial Factors Required for Transmission of Streptococcus pneumoniae in Mammalian Hosts.

ABSTRACT: The capacity of Streptococcus pneumoniae to successfully transmit and colonize new human hosts is a critical aspect of pneumococcal population biology and a prerequisite for invasive disease. However, the bacterial mechanisms underlying this process remain largely unknown. To identify bacterial factors required for transmission, we conducted a high-throughput genetic screen with a transposon sequencing (Tn-seq) library of a pneumococcal strain in a ferret transmission model. Key players in both metabolism and transcriptional regulation were identified as required for efficient bacterial transmission. Targeted deletion of the putative C3-degrading protease CppA, iron transporter PiaA, or competence regulatory histidine kinase ComD significantly decreased transmissibility in a mouse model, f

SUBMITTER: Rowe HM 

PROVIDER: S-EPMC6598203 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature