An improved method for identifying SUMOylation sites of viral proteins.
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ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC6598889 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
An improved method for identifying SUMOylation sites of viral proteins.
Liu Yan Y Tan Zhongyuan Z Shu Bo B Zhang Yuan Y Zheng Caishang C Ke Xianliang X Chen Xi X Wang Hanzhong H Zheng Zhenhua Z
Virologica Sinica 20171201 6
In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. Furthermore, this method provides an option for rapid and accurate identification of the potential viral protein SUMOylation sites. ...[more]
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