Intracellular Zn²⁺ transients modulate global gene expression in dissociated rat hippocampal neurons.

ABSTRACT: Zinc (Zn²⁺) is an integral component of many proteins and has been shown to act in a regulatory capacity in different mammalian systems, including as a neurotransmitter in neurons throughout the brain. While Zn²⁺ plays an important role in modulating neuronal potentiation and synaptic plasticity, little is known about the signaling mechanisms of this regulation. In dissociated rat hippocampal neuron cultures, we used fluorescent Zn²⁺ sensors to rigorously define resting Zn²⁺ levels and stimulation-dependent intracellular Zn²⁺ dynamics, and we performed RNA-Seq to characterize Zn²⁺-dependent transcriptional effects upon stimulation. We found that relatively small changes in cytosolic Zn²⁺ during stimulation altered

SUBMITTER: Sanford L 

PROVIDER: S-EPMC6598991 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature