Construction and analysis of degradome-dependent microRNA regulatory networks in soybean.

ABSTRACT:

Background

Usually the microRNA (miRNA)-mediated gene regulatory network (GRN) is constructed from the investigation of miRNA expression profiling and target predictions. However, the higher/lower expression level of miRNAs does not always indicate the higher/lower level of cleavages and such analysis, thus, sometimes ignores the crucial cleavage events. In the present work, the degradome sequencing data were employed to construct the complete miRNA-mediated gene regulatory network in soybean, unlike the traditional approach starting with small RNA sequencing data.

Results

We constructed the root-, cotyledon-, leaf- and seed-specific miRNA regulatory networks with the degradome sequencing data and the forthcoming verification of miRNA profiling analysis. As a result, we iden

SUBMITTER: Wang R 

PROVIDER: S-EPMC6599275 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature