Assessment of the impact of different fecal storage protocols on the microbiota diversity and composition: a pilot study.

ABSTRACT:

Background

Fecal samples are currently the most commonly studied proxy for gut microbiota. The gold standard of sample handling and storage for microbiota analysis is maintaining the cold chain during sample transfer and immediate storage at - 80 °C. Gut microbiota studies in large-scale, population-based cohorts require a feasible sample collection protocol. We compared the effect of three different storage methods and mock shipment: immediate freezing at - 80 °C, in 95% ethanol stored at room temperature (RT) for 48 h, and on blood collection card stored at RT for 48 h, on the measured composition of fecal microbiota of eight healthy, female volunteers by sequencing the V4 region of the 16S rRNA gene on an Illumina MiSeq.

Results

Shared operational taxonomic units (OTUs) b

SUBMITTER: Moossavi S 

PROVIDER: S-EPMC6599303 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature