Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks.

ABSTRACT:

Background

Anaplasma phagocytophilum is currently regarded as a single species. However, molecular studies indicate that it can be subdivided into ecotypes, each with distinct but overlapping transmission cycle. Here, we evaluate the interactions between and within clusters of haplotypes of the bacterium isolated from vertebrates and ticks, using phylogenetic and network-based methods.

Methods

The presence of A. phagocytophilum DNA was determined in ticks and vertebrate tissue samples. A fragment of the groEl gene was amplified and sequenced from qPCR-positive lysates. Additional groEl sequences from ticks and vertebrate reservoirs were obtained from GenBank and through literature searches, resulting in a dataset consisting of 1623 A. phagocytophilum field isolates. Phylogen

SUBMITTER: Jaarsma RI 

PROVIDER: S-EPMC6599317 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature