Single cell RNA-Seq reveals pre-cDCs fate determined by transcription factor combinatorial dose.

ABSTRACT:

Background

Classic dendritic cells (cDCs) play a central role in the immune system by processing and presenting antigens to activate T cells, and consist of two major subsets: CD141⁺ cDC (cDC1) and CD1c⁺ cDC (cDC2). A population of migratory precursor cells, the pre-cDCs, is the immediate precursors to both cDC subsets. Previous studies showed that there were two pre-committed pre-cDC subpopulations. However, the key molecular drivers of pre-commitment in human pre-cDCs were not investigated.

Results

To identify the key molecular drivers for pre-commitment in human pre-cDCs, we performed single cell RNA sequencing (RNA-Seq) of two cDC subsets and pre-cDCs, and bulk RNA-Seq of pre-cDCs and cDCs from human peripheral blood. We found that pre-DC subpopulat

SUBMITTER: Ma W 

PROVIDER: S-EPMC6599345 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature