Validation of endogenous reference genes in rat cerebral cortex for RT-qPCR analyses in developmental toxicity studies.

ABSTRACT: Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must be stably expressed across all the tested samples. Here, we have tested 10 common RGs for their expression stability in cerebral cortex from young rats after in utero exposure to thyroid hormone (TH) disrupting compounds. We found that all 10 RGs were stable according to the three algorithms geNorm, NormFinder and BestKeeper. The downstream target gene Pvalb was significantly downregulated in brains from young rats after in utero exposure to propylthiouracil (PTU), a medicinal drug inhibit

SUBMITTER: Ramhoj L 

PROVIDER: S-EPMC6599449 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature