Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution.

ABSTRACT:

Background

Sucrose is an attractive industrial carbon source due to its abundance and the fact that it can be cheaply generated from sources such as sugarcane. However, only a few characterized Escherichia coli strains are able to metabolize sucrose, and those that can are typically slow growing or pathogenic strains.

Methods

To generate a platform strain capable of efficiently utilizing sucrose with a high growth rate, adaptive laboratory evolution (ALE) was utilized to evolve engineered E. coli K-12 MG1655 strains containing the sucrose utilizing csc genes (cscB, cscK, cscA) alongside the native sucrose consuming E. coli W.

Results

Evolved K-12 clones displayed an increase in growth and sucrose uptake rates of 1.72- and 1.40-fold on sugarcane juice as compared to th

SUBMITTER: Mohamed ET 

PROVIDER: S-EPMC6599523 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature