A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides.

ABSTRACT:

Background

Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and resources. However, there is a lack of characterized promoters to drive expression of heterologous genes for strain engineering in R. toruloides.

Results

This data describes a set of native R. toruloides promoters, characterized over time in four different media commonly used for cultivation of this yeast. The promoter sequences were selected using transcriptional analysis and several of them were found to drive expression bidirectionally. Promoter expression strength was determined by measurement of EGFP and mRuby2 reporters by flow cytometry. A total of 2

SUBMITTER: Nora LC 

PROVIDER: S-EPMC6599526 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature