Host-derived fecal microRNAs can indicate gut microbiota healthiness and ability to induce inflammation.

ABSTRACT: Disruption of intestine-microbiota symbiosis can result in chronic gut inflammation. We hypothesize that assessing the initial inflammatory potential of the microbiota in patients is essential and that host-derived miRNAs, which can be found in feces, could fulfill this function. We investigated whether the gut microbiota composition impacts the fecal miRNA profile and thereby indicates its ability to influence intestinal inflammation. Methods: We used high-throughput qPCR to compare fecal miRNA profile between germ-free and conventional mice. Conventionalization of germfree mice by various colitogenic and non-colitogenic microbiotas (IL10^-/- and TLR5^-/- associated microbiota) was performed. Results: We identified 12 fecal miRNAs impacted by the presence

SUBMITTER: Viennois E 

PROVIDER: S-EPMC6599659 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature