Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity.

ABSTRACT: Influenza virus-infected cells vary widely in their expression of viral genes and only occasionally activate innate immunity. Here, we develop a new method to assess how the genetic variation in viral populations contributes to this heterogeneity. We do this by determining the transcriptome and full-length sequences of all viral genes in single cells infected with a nominally "pure" stock of influenza virus. Most cells are infected by virions with defects, some of which increase the frequency of innate-immune activation. These immunostimulatory defects are diverse and include mutations that perturb the function of the viral polymerase protein PB1, large internal deletions in viral genes, and failure to express the virus's interferon antagonist NS1. However, immune activation remains stocha

SUBMITTER: Russell AB 

PROVIDER: S-EPMC6600203 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature