Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B.

ABSTRACT: Orally administered fungal vaccines show promise for the prevention of infectious diseases. Edible mushrooms are deemed appropriate hosts to produce oral vaccines due to their low production cost and low risk of gene contamination. However, their low expression level of antigens has limited the potential development of oral vaccines using mushrooms. The low expression level might result from impurity of the transgenic mycelia since dikaryotic mycelia are commonly used as transformation materials. In this study, stable transgenic hepatitis B virus surface antigen (HBsAg) in Flammulina velutipes transformants was obtained by Agrobacterium-mediated transformation, followed by fruiting and basidiospore mating. The formation of HBsAg was detected by western blot analysis. The expr

SUBMITTER: Huang LH 

PROVIDER: S-EPMC6600300 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature