Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions.

ABSTRACT: The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of joint diseases. A number of protocols of in vitro differentiation of iPSC in chondrocytes for regenerative purposes have been recently described. However, in order to use these cells in clinics, the elimination of animal serum and feeder cells is essential. In our study, a strictly defined and controllable protocol was designed for the differentiation of pluripotent stem cells (BG01V, ND 41658*H, GPCCi001-A) in chondrocyte-like cells in serum- and a feeder cell-free system, using the embryoid bodies step. The extension of the protocol and culture conditi

SUBMITTER: Lach MS 

PROVIDER: S-EPMC6600514 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature