The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis.

ABSTRACT: Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these proteins to function effectively there needs to be structure within the PM allowing, for example, proteins in the same signaling cascade to be spatially organized. Here we demonstrate that several proteins with divergent functions are located in clusters of differing size in the membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals that these proteins move at different rates within the membrane. Actin and microtubule cytoskeletons appear to significantly regulate the mobility of one of these proteins (the pathogen receptor FLS2) and we further demonstrate that the cell wall is critical

SUBMITTER: McKenna JF 

PROVIDER: S-EPMC6601011 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature