High-throughput Exploration of the Network Dependent on AKT1 in Mouse Ovarian Granulosa Cells.

ABSTRACT: The PI3K/AKT signaling pathway is known to regulate a broad range of cellular processes, and it is often altered in several types of cancers. Recently, somatic AKT1 mutations leading to a strong activation of this kinase have been reported in juvenile granulosa cell tumors. However, the molecular role of AKT1 in the supporting cell lineage of the ovary is still poorly understood. To get insights into its function in such cells, we depleted Akt1 in murine primary granulosa cells and assessed the molecular consequences at both the transcript and protein levels. We were able to corroborate the involvement of AKT1 in the regulation of metabolism, apoptosis, cell cycle, or cytoskeleton dynamics in this ovarian cell type. Consistently, we showed in established granulosa cells that

SUBMITTER: Elzaiat M 

PROVIDER: S-EPMC6601207 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature