TMT Labeling for the Masses: A Robust and Cost-efficient, In-solution Labeling Approach.

ABSTRACT: Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quantification of single peptides across samples. However, such studies often require labeling of hundreds of micrograms of peptides such that the cost for labeling reagents represents a major contribution to the overall cost of an experiment. Here, we describe and evaluate a robust and cost-effective protocol for TMT labeling that reduces the quantity of required labeling reagent by a factor of eight and achieves complete labeling. Under- and overlabeling of peptides derived from complex digests of

SUBMITTER: Zecha J 

PROVIDER: S-EPMC6601210 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature