The mRNA-bound Proteome of Leishmania mexicana: Novel Genetic Insight into an Ancient Parasite.

ABSTRACT: Leishmania parasite infections, termed the leishmaniases, cause significant global infectious disease burden. The lifecycle of the parasite embodies three main stages that require precise coordination of gene regulation to survive environmental shifts between sandfly and mammalian hosts. Constitutive transcription in kinetoplastid parasites means that gene regulation is overwhelmingly reliant on post-transcriptional mechanisms, yet strikingly few Leishmania trans-regulators are known. Using optimized crosslinking and deep, quantified mass spectrometry, we present a comprehensive analysis of 1400 mRNA binding proteins (mRBPs) and whole cell proteomes from the three main Leishmania lifecycle stages. Supporting the validity, although the crosslinked RBPome is magnitudes m

SUBMITTER: de Pablos LM 

PROVIDER: S-EPMC6601212 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature