Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation.

ABSTRACT: Rapidly proliferating cells reshape their metabolism to satisfy their ever-lasting need for cellular building blocks. This phenomenon is exemplified in certain malignant conditions such as cancer but also during embryonic development when cells rely heavily on glycolytic metabolism to exploit its metabolic intermediates for biosynthetic processes. How cells reshape their metabolism is not fully understood. Here we report that loss of cathepsin L (Cts L) is associated with a fast proliferation rate and enhanced glycolytic metabolism that depend on lactate dehydrogenase A (LDHA) activity. Using mass spectrometry analysis of cells treated with a pan cathepsin inhibitor, we observed an increased abundance of proteins involved in central carbon metabolism. Further inspection of putative Cts L t

SUBMITTER: Weiss-Sadan T 

PROVIDER: S-EPMC6601214 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature