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Relating Structure and Dynamics in RNA Biology.

ABSTRACT: Recent advances in structural biology methods have enabled a surge in the number of RNA and RNA-protein assembly structures available at atomic or near-atomic resolution. These complexes are often trapped in discrete conformational states that exist along a mechanistic pathway. Single-molecule fluorescence methods provide temporal resolution to elucidate the dynamic mechanisms of processes involving complex RNA and RNA-protein assemblies, but interpretation of such data often requires previous structural knowledge. Here we highlight how single-molecule tools can directly complement structural approaches for two processes--translation and reverse transcription-to provide a dynamic view of molecular function.

SUBMITTER: Larsen KP

PROVIDER: S-EPMC6601459 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Relating Structure and Dynamics in RNA Biology.

Larsen Kevin P KP Choi Junhong J Prabhakar Arjun A Puglisi Elisabetta Viani EV Puglisi Joseph D JD

Cold Spring Harbor perspectives in biology 20190701 7

Recent advances in structural biology methods have enabled a surge in the number of RNA and RNA-protein assembly structures available at atomic or near-atomic resolution. These complexes are often trapped in discrete conformational states that exist along a mechanistic pathway. Single-molecule fluorescence methods provide temporal resolution to elucidate the dynamic mechanisms of processes involving complex RNA and RNA-protein assemblies, but interpretation of such data often requires previous s ...[more]

PMID: 31262948

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