Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model.

ABSTRACT:

Background

Patients with primary and metastatic brain cancer have an extremely poor prognosis, mostly due to the late diagnosis of disease. Urine, which lacks homeostatic mechanisms, is an ideal biomarker source that accumulates early and highly sensitive changes to provide information about the early stage of disease.

Methods

A rat model mimicking the local tumor growth process in the brain was established with intracerebral Walker 256 (W256) cell injection. Urine samples were collected on days 3, 5, and 8 after injection, and then analyzed by liquid chromatography coupled with tandem mass spectrometry.

Results

In the intracerebral W256 model, no obvious clinical manifestations or abnormal magnetic resonance imaging (MRI) signals were found on days 3 or 5; at these t

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6601583 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature