Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.

ABSTRACT: Auditory signals are processed in multiple central nervous system structures, including the auditory cortex (AC). Development of stem cell biology provides the opportunity to identify neural stem cells (NSCs) in the central nervous system. However, it is unclear whether NSCs exist in the AC. The aim of this study is to determine the existence of NSCs in the postnatal mouse AC. To accomplish this aim, postnatal mouse AC tissues were dissected and dissociated into singular cells and small cell clumps, which were suspended in the culture medium to observe neurosphere formation. The spheres were examined by quantitative real-time polymerase chain reaction and immunofluorescence to determine expression of NSC genes and proteins. In addition, AC-spheres were cultured in the presence or absence o

SUBMITTER: Hu Z 

PROVIDER: S-EPMC6602114 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature