Ontology highlight
ABSTRACT:
SUBMITTER: Megyeri M
PROVIDER: S-EPMC6602303 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Journal of cell science 20190624 12
<i>LAG1</i> was the first longevity assurance gene discovered in <i>Saccharomyces cerevisiae</i> The Lag1 protein is a ceramide synthase and its homolog, Lac1, has a similar enzymatic function but no role in aging. Lag1 and Lac1 lie in an enzymatic branch point of the sphingolipid pathway that is interconnected by the activity of the C4 hydroxylase, Sur2. By uncoupling the enzymatic branch point and using lipidomic mass spectrometry, metabolic labeling and <i>in vitro</i> assays we show that Lag ...[more]