Sensitive bioluminescence imaging of fungal dissemination to the brain in mouse models of cryptococcosis.

ABSTRACT: Cryptococcus neoformans is a leading cause of fungal brain infection, but the mechanism of dissemination and dynamics of cerebral infection following pulmonary disease are poorly understood. To address these questions, non-invasive techniques that can study the dynamic processes of disease development and progression in living animal models or patients are required. As such, bioluminescence imaging (BLI) has emerged as a powerful tool to evaluate the spatial and temporal distribution of infection in living animals. We aimed to study the time profile of the dissemination of cryptococcosis from the lung to the brain in murine models by engineering the first bioluminescent C. neoformans KN99α strain, expressing a sequence-optimized red-shifted luciferase. The high pathogen speci

SUBMITTER: Vanherp L 

PROVIDER: S-EPMC6602310 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature