Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord.

ABSTRACT: The coordinated spatial and temporal regulation of gene expression in the vertebrate neural tube determines the identity of neural progenitors and the function and physiology of the neurons they generate. Progress has been made deciphering the gene regulatory programmes that are responsible for this process; however, the complexity of the tissue has hampered the systematic analysis of the network and the underlying mechanisms. To address this, we used single cell mRNA sequencing to profile cervical and thoracic regions of the developing mouse neural tube between embryonic days 9.5-13.5. We confirmed that the data accurately recapitulates neural tube development, allowing us to identify new markers for specific progenitor and neuronal populations. In addition, the analysis highlighted a pre

SUBMITTER: Delile J 

PROVIDER: S-EPMC6602353 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature