Volumetric Ca²⁺ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy.

ABSTRACT: Calcium imaging using two-photon scanning microscopy has become an essential tool in neuroscience. However, in its typical implementation, the tradeoffs between fields of view, acquisition speeds, and depth restrictions in scattering brain tissue pose severe limitations. Here, using an integrated systems-wide optimization approach combined with multiple technical innovations, we introduce a new design paradigm for optical microscopy based on maximizing biological information while maintaining the fidelity of obtained neuron signals. Our modular design utilizes hybrid multi-photon acquisition and allows volumetric recording of neuroactivity at single-cell resolution within up to 1 × 1 × 1.22 mm volumes at up to 17 Hz in awake behaving mice. We establish the capabilities and potential of the

SUBMITTER: Weisenburger S 

PROVIDER: S-EPMC6602798 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature