Comparison of different methods for measuring the superoxide radical by EPR spectroscopy in buffer, cell lysates and cells.

ABSTRACT: As superoxide anion is of keen interest in biomedical research, it is highly desirable to have a technique allowing its detection sensitively and specifically in biological media. If electron paramagnetic resonance (EPR) techniques and probes have been individually described in the literature, there is actually no comparison of these techniques in the same conditions that may help guiding researchers for selecting the most appropriate approach. The aim of the present study was to compare different EPR strategies in terms of sensitivity and specificity to detect superoxide (vs. hydroxyl radical). Three main classes of EPR probes were used, including paramagnetic superoxide scavengers (such as nitroxides TEMPOL and mitoTEMPO as well as trityl CT-03), a spin trap (DIPPMPO), and diamagnetic su

SUBMITTER: Scheinok S 

PROVIDER: S-EPMC6602867 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature