Molecular mechanism of FSHR expression induced by BMP15 in human granulosa cells.

ABSTRACT:

Purpose

Follicle-stimulating hormone receptor (FSHR) expression in granulosa cells is critical in enabling follicles to achieve accelerated growth. Although FSHR expression has been reported to be epigenetically regulated, the mechanism is unclear. Cooperation between oocytes and granulosa cells is also essential for normal follicular growth. Among oocyte-derived factors, bone morphogenetic protein 15 (BMP15) promotes follicular growth and is suggested to have epigenetic effects. We examined the role of BMP15 in the acquirement of FSHR in human granulosa cells.

Methods

Immortalized non-luteinized human granulosa (HGrC1) cells were stimulated with trichostatin A (TSA) or BMP15 to analyze FSHR expression, histone modifications, and USF1/2 binding at the FSHR promoter region. H

SUBMITTER: Shimizu K 

PROVIDER: S-EPMC6603124 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature