A real-time PCR screening assay for the universal detection of lumpy skin disease virus DNA.

ABSTRACT:

Objective

The resurgence of lumpy skin disease virus isolates of different genotypic natures abolishes the accuracy of assays that target either vaccine or field strain genome. The aim of the present study was to develop a universal real-time PCR assay using TaqMan chemistry to cover field, vaccine, and recombinant strains of lumpy skin disease virus isolates.

Results

The PCR assay was designed based on a LSDV044 target region that offers a unique identification locus to facilitate the sensitive and specific detection of all isolates known to date. The efficiency of amplification, determined over five orders of magnitude, was 93%, with the standard deviation remaining in the range of 0.11-0.23. Evaluation of the assay repeatability on three different days revealed that the i

SUBMITTER: Alexander S 

PROVIDER: S-EPMC6604310 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature