Organic solvent modifier and temperature effects in non-aqueous size-exclusion chromatography on reversed-phase columns.

ABSTRACT: Common reversed-phase columns (C₁₈, C₄, phenyl, and cyano) offer inert surfaces suitable for the analysis of polymers by size-exclusion chromatography (SEC). The effect of tetrahydrofuran (THF) solvent and the mixtures of THF with a variety of common solvents used in high performance liquid chromatography (acetonitrile, methanol, dimethylformamide, 2-propanol, ethanol, acetone and chloroform) on reversed-phase stationary phase characteristics relevant to size exclusion were studied. The effect of solvent on the elution of polystyrene (PS) and poly(methyl methacrylate) (PMMA) and the effect of column temperature (within a relatively narrow range corresponding to typical chromatographic conditions, i.e., 10°C-60°C) on the SEC partition coefficients K_SEC of PS

SUBMITTER: Caltabiano AM 

PROVIDER: S-EPMC6604611 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature