N-(2-(Dimethylamino)Ethyl)-4-¹⁸F-Fluorobenzamide: A Novel Molecular Probe for High-Contrast PET Imaging of Malignant Melanoma.

ABSTRACT: Malignant melanoma is an aggressive and serious form of skin cancer, with prognosis and treatment outcome depending heavily on the clinical stage of the disease at the time of diagnosis. Here, we synthesized a novel ¹⁸F-labeled benzamide derivative to target melanoma and then evaluated its biologic characteristics in small-animal models. Methods:N-(2-(dimethylamino)ethyl)-4-¹⁸F-fluorobenzamide (¹⁸F-DMFB) was synthesized by reaction of N-succinimidyl 4-¹⁸F-fluorobenzoate with N,N-dimethylethylenediamine. The binding affinity of ¹⁸F-DMFB was measured in B16F10 (mouse melanoma) cells with or without l-tyrosine. Small-animal PET imaging with ¹⁸F-DMFB was performed on B16F10 xenograft and metastasis mo

SUBMITTER: Pyo A 

PROVIDER: S-EPMC6604686 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature