Interleukin-6-mediated CCR9⁺ interleukin-17-producing regulatory T cells polarization increases the severity of necrotizing enterocolitis.

ABSTRACT:

Background

Increased frequency of CCR9⁺ CD4⁺ T cells in peripheral blood is linked to several gastrointestinal inflammatory diseases; however, its relationship with necrotizing enterocolitis (NEC) is not understood. We investigated whether the frequencies of CCR9⁺ CD4⁺ T cells and related subsets were increased in peripheral blood of both patients and mice with NEC.

Methods

CCR9⁺ CD4⁺ T cells and related subsets were evaluated by flow cytometry in peripheral blood collected from both patients and mice with NEC and controls. The suppressive function of CCR9⁺ regulatory T (Treg) cells in NEC was assessed via in vitro suppression assay. An in vitro T cell polarization assay was performed to investigate

SUBMITTER: Ma F 

PROVIDER: S-EPMC6604880 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature