Uptake and reduction of Se(IV) in two heterotrophic aerobic Pseudomonads strains isolated from boreal bog environment.

ABSTRACT: Selenite (Se(IV), SeO₃ ^2-) uptake and the effect of selenite supplement on protein synthesis was investigated in two Pseudomonas sp. strains isolated from a boreal bog. These aerobic bacteria efficiently reduced Se(IV) with intracellular reduced Se⁰ observed in the cytoplasm under dark aerobic conditions. The proteome analysis of Se(IV) supplement and temperature responses by SDS-PAGE gel electrophoresis showed variations in the protein expression on the 40-60 kDa regions following these stress factors, probably through enzymes associated to oxidative stress or temperature adaptation. NO₃ ^-/NO₂ ^-/SO₄ ^2- addition enhanced Se(IV) uptake in both bacteria, but Se(IV) uptake sustained also

SUBMITTER: Lusa M 

PROVIDER: S-EPMC6604954 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature