High-throughput environmental DNA analysis informs a biological assessment of an urban stream.

ABSTRACT: There is growing interest in the use of DNA barcoding and metabarcoding approaches to aid biological assessments and monitoring of waterbodies. While biodiversity measured by morphology and by DNA often has been found correlated, few studies have compared DNA data to established measures of impairment such as multimetric pollution tolerance indices used by many bioassessment programs. We incorporated environmental DNA (eDNA) metabarcoding of seston into a rigorous watershed-scale biological assessment of an urban stream to examine the extent to which eDNA richness and diversity patterns were correlated with multimetric indices and ecological impairment status designations. We also evaluated different filtering approaches and taxonomic classifications to identify best practices for environm

SUBMITTER: Bagley M 

PROVIDER: S-EPMC6605098 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature